In the spring of 1965, within weeks of 3,500 American Marines arriving in Vietnam, a 39-year-old Briton named Larry Burrows began work on a feature for LIFE magazine, chronicling the day-to-day experience of U.S. troops on the ground – and in the air – in the midst of the rapidly widening war. The photographs in this gallery focus on a calamitous March 31, 1965, helicopter mission; Burrows’ “report from Da Nang”, featuring his pictures and his personal account of the harrowing operation, was published two weeks later as a now-famous cover story in the April 16, 1965, issue of LIFE.

Photo: Man your battle stations: The crew chief of helicopter Yankee Papa 13, lance corporal James C. Farley, mans an M-60 machine gun during a mission near Da Nang, Vietnam on March 31, 1965. (Photo by Larry Burrows/Time & Life Pictures)

A Sumatran Tiger looks at visiting children from it's enclosure during the ZSL London Zoo's annual stocktake of animals on January 5, 2015 in London, England. The zoo's annual stocktake requires keepers to check on the numbers of every one of the 800 different animal species, including every invertebrate, bird, fish, mammal, reptile, and amphibian. (Photo by Dan Kitwood/Getty Images)

The entire cast perform during a production media call for Singin' In The Rain at Her Majesty's Theatre on May 12, 2016 in Melbourne, Australia. (Photo by Robert Cianflone/Getty Images)

“Honorable Mention”. An Indian rhinoceros, far from home and stuck inside with late-winter blues at the Toronto zoo. Photo location: Toronto, Ontario, Canada. (Photo and caption by Stephen De Lisle/National Geographic Photo Contest)

India: A sweeper cleans the side of a road near a replica of the Eiffel Tower amid heavy fog in the early morning in the northern Indian city of Chandigarh January 18, 2010. (Photo by Ajay Verma/Reuters)

This picture taken on March 1, 2016 shows a man running a mobile pig mating service and waiting outside a customer's home with his male pig in a cage pulled behind his motorcycle in the northern province of Bac Ninh. (Photo by Hoang Dinh Nam/AFP Photo)

A vendor sleeps at his vegetables shop at a main market in Colombo, Sri Lanka May 31, 2016. (Photo by Dinuka Liyanawatte/Reuters)

Laura Giombini of Italy reacts during the Women's Beach Volleyball preliminary round Pool D match against Jamie Lynn Broder and Kristina Valjas of Canada on Day 2 of the Rio 2016 Olympic Games at the Beach Volleyball Arena on August 7, 2016 in Rio de Janeiro, Brazil. (Photo by Shaun Botterill/Getty Images)