In the spring of 1965, within weeks of 3,500 American Marines arriving in Vietnam, a 39-year-old Briton named Larry Burrows began work on a feature for LIFE magazine, chronicling the day-to-day experience of U.S. troops on the ground – and in the air – in the midst of the rapidly widening war. The photographs in this gallery focus on a calamitous March 31, 1965, helicopter mission; Burrows’ “report from Da Nang”, featuring his pictures and his personal account of the harrowing operation, was published two weeks later as a now-famous cover story in the April 16, 1965, issue of LIFE.

Photo: Man your battle stations: The crew chief of helicopter Yankee Papa 13, lance corporal James C. Farley, mans an M-60 machine gun during a mission near Da Nang, Vietnam on March 31, 1965. (Photo by Larry Burrows/Time & Life Pictures)

A three-month-old Sumatran tiger cub named “Bandar” shows his displeasure after being dunked in the tiger exhibit moat for a swim reliability test at the National Zoo in Washington, on November 6, 2013. All cubs born at the zoo must take a swim test before being allowed to roam in the exhibit. Bandar passed his test. (Photo by Manuel Balce Ceneta/Associated Press)

Karis, an eleven week old lion cub, plays in fallen leaves brushed up by keepers in her enclosure at Blair Drummond Safari Park, near Stirling, Scotland, on November 20, 2013. (Photo by Andrew Milligan/PA Wire)

Sarah Paulson and Amanda Peet arrive at the 20th annual Screen Actors Guild Awards. (Photo by Jordan Strauss/AP Photo/Invision)

A fellow officer comes to the aid of injured patrolman C.V. Satt, who was pelted with rocks and beer bottles during a clash between police and relief demonstrators in Denver September 23, 1935. (Photo by Henry G. Eisenhand/AP Photo)

Victoria's Secret Angel Martha Hunt celebrates the 2015 What Is s*xy? List in San Diego on September 26, 2015 in San Diego, California. (Photo by Joe Scarnici/Getty Images for Victoria's Secret)

A man is silhouetted as he stands on a walkway in the sky lobby at the Chancellery, the seat of German Chancellor Angela Merkel (not pictured) in Berlin, May 7, 2014. (Photo by Thomas Peter/Reuters)

A participant runs in high-heels as she competes in the Stiletto Run in Bucharest June 14, 2014. The annual 50 metres race requires participants to wear high-heels that are at least 7cm tall. (Photo by Bogdan Cristel/Reuters)