In the spring of 1965, within weeks of 3,500 American Marines arriving in Vietnam, a 39-year-old Briton named Larry Burrows began work on a feature for LIFE magazine, chronicling the day-to-day experience of U.S. troops on the ground – and in the air – in the midst of the rapidly widening war. The photographs in this gallery focus on a calamitous March 31, 1965, helicopter mission; Burrows’ “report from Da Nang”, featuring his pictures and his personal account of the harrowing operation, was published two weeks later as a now-famous cover story in the April 16, 1965, issue of LIFE.

Photo: Man your battle stations: The crew chief of helicopter Yankee Papa 13, lance corporal James C. Farley, mans an M-60 machine gun during a mission near Da Nang, Vietnam on March 31, 1965. (Photo by Larry Burrows/Time & Life Pictures)

Brain-on-a-chip. Dazzling in green and magenta this image shows the nerve fibres (in green) produced by neural stem cells (in magenta) as they grow on a synthetic gel. Captured by a technique known as confocal microscopy, the image is part of research shedding light on how tinkering with the environment can affect the way in which nerve fibres grow. (Photo by Collin Edington and Iris Lee/Massachusetts Institute of Technology/Wellcome Images)

Christian Bale poses for a fan at the 25th annual Screen Actors Guild Awards at the Shrine Auditorium & Expo Hall on Sunday, January 27, 2019, in Los Angeles. (Photo by Mario Anzuoni/Reuters)

A woman tries to catch snowflakes with her tongue during a snowfall on Chandragiri Hills in Kathmandu, Nepal on January 23, 2019. (Photo by Navesh Chitrakar/Reuters)

A boy rides on a snow wagon towed by a robot dressed as a Teddy bear, which only moves forward by moving its legs, during the Ice and snow carnival at Taoranting park in Beijing February 9, 2015. (Photo by Kim Kyung-Hoon/Reuters)

Sea lion “Jay” paints a Chinese character reading “the Serpent” at the Hakkeijima Sea Paradise aquarium in Yokohama, suburban Tokyo on January 3, 2013. The event, marking the forthcoming Chinese lunar calender year of the snake was held as part of a New Year's attraction. (Photo by Kazuhiro Nogi/AFP Photo)

A mother bear takes a dip in the water to cool off in the warm summer sun but refuses to leave her three youngsters behind – so they climb aboard her back. (Photo by Jon Langeland/Solent News & Photo Agency)

Peru, Iquitos. Restaurant in Bellavista, an area by the Amazon on April 2005. (Photo by Jean-Claude Coutausse)