In the spring of 1965, within weeks of 3,500 American Marines arriving in Vietnam, a 39-year-old Briton named Larry Burrows began work on a feature for LIFE magazine, chronicling the day-to-day experience of U.S. troops on the ground – and in the air – in the midst of the rapidly widening war. The photographs in this gallery focus on a calamitous March 31, 1965, helicopter mission; Burrows’ “report from Da Nang”, featuring his pictures and his personal account of the harrowing operation, was published two weeks later as a now-famous cover story in the April 16, 1965, issue of LIFE.

Photo: Man your battle stations: The crew chief of helicopter Yankee Papa 13, lance corporal James C. Farley, mans an M-60 machine gun during a mission near Da Nang, Vietnam on March 31, 1965. (Photo by Larry Burrows/Time & Life Pictures)

Gunfire erupted at a Denver pot celebration Saturday, injuring two people and scattering a crowd of thousands who had gathered for the first 4/20 counterculture holiday since the state legalized marijuana... Photo: A woman smokes a large marijuana joint at the Vancouver Art Gallery during the annual 4/20 day, which promotes the use of marijuana, in Vancouver, British Columbia April 20, 2013. (Photo by Ben Nelms/Reuters)

1st prize in the People Observed Portraits Stories category. Carla Kogelman, the Netherlands. The photo shows Hannah and Alena, two sisters living in the rural village of Merkenbrechts, Austria. (Photo by Carla Kogelman/World Press Photo)

Three prostitutes lean out of a window in Santiago, Chile, in an attempt to entice customers inside, circa 1950. (Photo by Three Lions/Getty Images)

Robert Burck, a street performer known as “The Naked Cowboy” dips a woman as he poses for a photo in Times Square in New York, U.S., August 4, 2016. (Photo by Carlo Allegri/Reuters)

A man dressed as the “Star Wars” character Darth Vader sits on a bench in central Lviv, Ukraine, November 24, 2016. (Photo by Gleb Garanich/Reuters)

A Vietnamese bride is seen pushing the groom on a scooter during a photo shoot for their wedding in An Bang Beach outside Hoi An in Vietnam December 11, 2016. (Photo by Reuters/Stringer)

A sеx worker participates in a march to raise public awareness on human rights issues in their profession on International Day to End Violence Against Sеx Workers in Skopje, Macedonia December 17, 2016. (Photo by Ognen Teofilovski/Reuters)