In the spring of 1965, within weeks of 3,500 American Marines arriving in Vietnam, a 39-year-old Briton named Larry Burrows began work on a feature for LIFE magazine, chronicling the day-to-day experience of U.S. troops on the ground – and in the air – in the midst of the rapidly widening war. The photographs in this gallery focus on a calamitous March 31, 1965, helicopter mission; Burrows’ “report from Da Nang”, featuring his pictures and his personal account of the harrowing operation, was published two weeks later as a now-famous cover story in the April 16, 1965, issue of LIFE.

Photo: Man your battle stations: The crew chief of helicopter Yankee Papa 13, lance corporal James C. Farley, mans an M-60 machine gun during a mission near Da Nang, Vietnam on March 31, 1965. (Photo by Larry Burrows/Time & Life Pictures)

A three-month-old Sumatran tiger cub named “Bandar” shows his displeasure after being dunked in the tiger exhibit moat for a swim reliability test at the National Zoo in Washington, on November 6, 2013. All cubs born at the zoo must take a swim test before being allowed to roam in the exhibit. Bandar passed his test. (Photo by Manuel Balce Ceneta/Associated Press)

Karis, an eleven week old lion cub, plays in fallen leaves brushed up by keepers in her enclosure at Blair Drummond Safari Park, near Stirling, Scotland, on November 20, 2013. (Photo by Andrew Milligan/PA Wire)

Sarah Paulson and Amanda Peet arrive at the 20th annual Screen Actors Guild Awards. (Photo by Jordan Strauss/AP Photo/Invision)

Confiscated rare and protected wildlife products such as these stuffed Sumatran tiger skins are burned and destroyed during a ceremony by government forestry and wildlife officials in Banda Aceh, Aceh province, Indonesia May 23, 2016 in this photo taken by Antara Foto. (Photo by Irwansyah Putra/Reuters/Antara Foto)

In this September 4, 1963, file photo, a police officer carries off a demonstrator holding a Confederate flag after a group of demonstrators protested enrollment of two African-Americans at Ramsay High School in Birmingham, Ala. The Confederate battle flag has been removed from South Carolina's Statehouse grounds, in the wake of the massacre of nine African-Americans, including a state senator, at an historic black church in Charleston in June 2015. (Photo by AP Photo)

Fat Jew makes his entrance at the first birthday party for White Girl Rosé hosted by Elite Daily at The Dream Downtown, Sunday, July 17, 2016, in New York. (Photo by Diane Bondareff/Invision for White Girl Rosé/AP Images)